Description
CST's PathScan® Total Smad2 Sandwich ELISA Kit #7244 detects endogenous levels of total Smad2 protein. As shown in Figure 1, both phospho- and nonphospho-Smad2 proteins from untreated and TGFβ treated MV1LU cell lysates are detected by this kit. In Figure 3, Western blot analysis of protein captured in the Smad2 Rabbit mAb #3109 coated microwell shows a single band corresponding to the Smad2 protein. Smad2 in Hela cells also can be detected by this kit (data not shown). This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species